Alleviation of Antioxidant Defense System by Ozonized Olive Oil in DNBS-Induced Colitis in Rats

نویسندگان

  • Eman Abu-Gharbieh
  • Fatehia A Bayoumi
  • Naglaa G Ahmed
چکیده

The aim of the study was to evaluate the potential protective effect of ozonized olive oil (OZO) in 2,4-dinitrobenzene sulphuric acid (DNBS) induced colitis in rats and to elucidate the role of some antioxidant defense system (superoxide dismutase "SOD," glutathione peroxidase "GSH-Px," and catalase "CAT") in these effects. The physicochemical parameters including viscosity, peroxide, and acid values of olive oil and OZO were evaluated. The animals were divided into several groups and the colitis was induced in the rats by intracolonic instillation of DNBS at dose of 15 mg/rat. Olive oil (OO) at dose of 6 mg/kg and OZO at doses of 3 and 6 mg/kg was administered orally for 7 days, starting the day before induction of colitis. Our results showed that macroscopic and microscopic damage scores were significantly reduced in a dose response manner in rats pretreated with OZO only. In contrast, CAT, GSH-Px, and SOD activities were significantly increased in the distal colon of inflamed animals pretreated with OZO with respect to control group dose dependently. Results demonstrate that OZO pretreatment exerts protective effects in DNBS induced colitis in rats and provide evidence that the protective effects of OZO are mediated by stimulation of some antioxidant enzymes.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

DNBS/TNBS colitis models: providing insights into inflammatory bowel disease and effects of dietary fat.

Inflammatory Bowel Diseases (IBD), including Crohn's Disease and Ulcerative Colitis, have long been associated with a genetic basis, and more recently host immune responses to microbial and environmental agents. Dinitrobenzene sulfonic acid (DNBS)-induced colitis allows one to study the pathogenesis of IBD associated environmental triggers such as stress and diet, the effects of potential thera...

متن کامل

Effect of Coriandrum sativum hydroalcoholic extract and its essential oil on acetic acid- induced acute colitis in rats

Objective: The aim of this study was to determine the protective effects of Coriandrum sativum on acetic acid-inducedcolitis in rats. C. sativum (Coriander) has long been used in Iranian traditional medicine and its use as an anti-inflammatory agent is still common in some herbal formulations.  Materials and Methods: Colitis was induced by intra-rectal administration of 2ml acetic acid 4% in fa...

متن کامل

Renoprotective Effects of Naringenin and Olive Oil against Cyclosporine- Induced Nephrotoxicity in Rats

Background: Prolonged use of cyclosporine A for prevention of allograft rejection is associated with nephrotoxicity development. Naringenin and olive oil are beneficial dietary antioxidants with potential renoprotective properties. This study aimed to evaluate the therapeutic effect of naringenin and olive oil in alleviating cyclosporine induced nephrotoxicity in rats by the assessment of renal...

متن کامل

Therapeutic Effects of Ozonized Olive Oil in the Treatment of Intractable Fistula and Wound after Surgical Operation

20 patients at several hospitals who underwent surgical operation between August 1998 to August 2000 had ozonized olive oil applied to their intractable fistula or wounds. The ozonized olive oil treatment was fully effective in 19 of the cases. The one exception was for a case of pilonidal sinus. Ozonized olive oil was especially effective in treating intractable abdominal fistula to digestive ...

متن کامل

An In Vitro Evaluation of Ozonized Organic Extra-Virgin Olive Oil on Giardia Lamblia Cysts

BACKGROUND Giardia lamblia is a common intestinal parasite that has been reported all over the world. OBJECTIVES This study was conducted to evaluate the effect of ozonized organic extra-virgin olive oil on the cyst of G. lamblia. METHODS The olive oil was ozonized based on international standards and confirmed by the world health organization (WHO) at various times in a generator. The ozon...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 2014  شماره 

صفحات  -

تاریخ انتشار 2014